Isolation of substances that stimulate RNA synthesis from red kidney bean: their activities in lymphocytes and Escherichia coli.
نویسندگان
چکیده
An extract from red kidney beans (Phaseolus vulgaris) was fractionated with respect to RNA synthesis-stimulating activity. The activity of the fractions was tested in chicken-spleen lymphocytes cultured in serum-free medium and in plasmolyzed E. coli cells. Lymphocyte-stimulating activity was found in a fraction called F III. This fraction contained protein and orcinol-positive carbohydrate. The protein (called F III p) responsible for the observed activity was separated from the contaminating carbohydrate by acid precipitation. F III p appears to belong to the group of well-known mitogens of high molecular weight present in red kidney beans. A bacteria-stimulating factor was found in a fraction called F V. This factor strongly stimulated RNA synthesis in the bacterial system, and had a weak stimulating effect in the lymphocyte system. In plasmolyzed E. coli cells, F V counteracted the inhibiting effect of rifampicin on RNA synthesis.
منابع مشابه
Sequential Microwave-assisted Extraction for Isolation of Quercetin from Red Kidney Bean
Quercetin is a biological flavonoid, which can be found in red kidney bean at high concentration. In this study, a comparison was conducted between traditional and modern extraction methods. Sequential microwave-assisted extraction was used for isolation of quercetin from red kidney bean. The effect of several factors such as particle size of grinded kidney bean, extraction solvent, microwave p...
متن کاملInhibitory Effect of Black and Red Pepper and Thyme Extracts and Essential Oils on Enterohemorrhagic Escherichia coli and DNase Activity of Staphylococcus aureus
In this study, extracts and essential oils of Black and Red pepper and Thyme were tested for antibacterial activity against Escherichia coli O157:H7 andStaphylococcus aureus. Black and Red pepper and Thyme were provided from Iranian agricultural researches center. 2 g of each plant powder was added to 10 cc ethanol 96°. After 24 h, the crude extract was separated as an alcoholic extract and con...
متن کاملInhibitory Effect of Black and Red Pepper and Thyme Extracts and Essential Oils on Enterohemorrhagic Escherichia coli and DNase Activity of Staphylococcus aureus
In this study, extracts and essential oils of Black and Red pepper and Thyme were tested for antibacterial activity against Escherichia coli O157:H7 andStaphylococcus aureus. Black and Red pepper and Thyme were provided from Iranian agricultural researches center. 2 g of each plant powder was added to 10 cc ethanol 96°. After 24 h, the crude extract was separated as an alcoholic extract and con...
متن کاملIsolation and expression of recombinant viral protein (VP2) from Iranian isolates of Infectious Pancreatic Necrosis Virus (IPNV) in Escherichia coli
Infectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae that has been linked to high mortalities in salmonids. Bacterial based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. VP2 is a structural viral protein of IPNV with immunogenicity effects. In this study IPNV was isolated from diseased fry ...
متن کاملConstruction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary
Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 2 شماره
صفحات -
تاریخ انتشار 1973